Research Assistant 1

Case Western Reserve University • Cleveland, OH • 1h ago

POSITION OBJECTIVE

Working under moderate supervision, perform research work in lipid nanoparticle (LNP) formulation and in vitro gene therapy experiments. The Research Assistant 1 will support the laboratory's mission of developing next-generation delivery systems for gene therapy. This position supports research aimed at developing effective non-viral gene therapies for serious lung diseases including cystic fibrosis and others. This position will work with animals.

ESSENTIAL FUNCTIONS

Perform research work using lab skills; key responsibilities include high-throughput lipid nanoparticle synthesis and characterization, cell culture maintenance (including air-liquid interface cultures and other primary cell models), and conducting assays in cell models and mice to evaluate nanoparticle performance. Synthesize lipid nanoparticles using microfluidic mixing and characterize formulations by dynamic light scattering (DLS), zeta potential measurement, and RNA encapsulation efficiency assays. Record results for experiments. Operate and maintain the lab's high-throughput LNP screening platform. (30%)

Assist in analyzing LNP samples using cultured cell lines (e.g., HeLa, HEK293, 16HBE) and primary airway epithelial cells using via luciferase assays, flow cytometry, and fluorescence microscopy. (25%)

Maintain detailed electronic lab notebooks documenting experimental procedures, formulation parameters, and results. Organize data using spreadsheet and graphing software to support manuscript preparation and lab meetings. (15%)

Perform molecular biology tasks including molecular cloning, genetic construct design, plasmid preparation, RNA quantification, gel electrophoresis, and PCR. Assist with protein expression and characterization for novel delivery modalities. (15%)

Maintain laboratory equipment utilized in experiments including the microfluidic mixer, DLS instrument, plate readers, and cell culture hoods. Coordinate instrument calibration and service as needed. (6%)

Order laboratory supplies including reagents, consumables, and lipid stocks. Track inventory and coordinate with vendors. (6%)

NONESSENTIAL FUNCTIONS

Perform other duties as assigned. (3%)

CONTACTS

Department: Frequent contact with the principal investigator and lab members to discuss experimental progress, troubleshoot protocols, and coordinate research activities.

University: Occasional contact with core facilities (flow cytometry, imaging, genomics) and other departments to coordinate shared resources and exchange information.

External: Occasional contact with reagent and equipment vendors to obtain quotes, place orders, and resolve supply issues.

Students: Regular contact with graduate and undergraduate students in the lab to provide guidance on techniques and exchange information.

SUPERVISORY RESPONSIBILITY

This position has no direct supervision of staff employees.

QUALIFICATIONS

Education/Experience: Bachelor's degree and 0 to 1 year of experience or Associate's degree in an approved biotechnology program and 0 to 2 years of experience required.

REQUIRED SKILLS

Has knowledge of commonly-used concepts, practices, and procedures in molecular biology and biochemistry.

Relies on instructions and pre-established guidelines to perform the functions of the job.

Ability to operate laboratory equipment including pipettes, centrifuges, plate readers, and cell culture hoods. Experience with DLS or microfluidic devices is a plus.

Demonstrated history of successful support, education, and advocacy for all students, aligned with the values, mission, and messaging of the university, while adhering to the staff policy on conflict of commitment and interest.

Willingness to learn and work with Artificial Intelligence (AI) tools and technologie

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