Job Family :
Scientist (Digital)
Travel Required :
None
Clearance Required :
Ability to Obtain Public Trust
What You Will Do :
We are currently searching for an Animal Biologist to provide services and deliverables through the Transgenic Core supporting the overall operational objectives of the National Heart, Lung, and Blood Institute (NHLBI). This is a full-time, on-site opportunity in Bethesda, MD.
Conduct behavioral testing and perform surgery on small animal models.
Coordinate husbandry, animal care and enrichment activities to maintain a clean, safe and regulatory compliant animal research program.
Conduct research and perform experiments on the selection and breeding of animals.
Work with staff to maintain, breed, geno@type and pheno@type transgenic animal colony; maintain records of animal colony.
Independently perform genotyping of animals.
Maintain animal lines in transgenic facilities.
Maintain animal colonies; handle animals for hormone injection, mating, timed mating, and other experimental procedures; handle, dissect and euthanize animals.
Work with staff on maintaining mouse colonies which may include setting up mating pairs and checking plugs, genotyping mice by tail biopsy, injecting mice with hormones or other compounds, injecting cells into immuno-compromised mice and monitoring and analyzing tumor growth.
Generate transgenic and knockout cell lines using microinjection method.
Perform simple surgeries on animals and skilled routine surgical procedures in support of on-going animal studies.
Handle experimental animals including tail vein injections, administration of anesthesia and monitoring of animal viral signs.
Prepare and analyze animal tissues; perform a variety of specialized tissue fixation, embedding, sectioning and staining techniques; perform experiments with primary tissues and cells in culture, utilizing sterile techniques.
Harvest and collect animal embryos, tissues, and organs.
Carry out molecular biological experiments, including extracting genomic DNA, performing PCR and gel electrophoresis, cloning DNA fragments and preparing plasmid DNA, generating high quality RNA using in-vitro transcription.
Perform tissue culture and related techniques including primary cells, such as human fibroblasts; maintain IPSC lines.
Perform standard molecular biology techniques, including, but not limited to, cloning, in situ hybridization and qPCR.
Maintain cell lines and perform basic cell culture assays, including, but not limited to, transfection and immunocytochemistry.
Perform immunohistochemistry on animal tissues; carry out molecular biological experiments, including extracting genomic DNA, performing PCR and gel electrophoresis, cloning DNA fragments and preparing plasmid DNA, generating high quality RNA using in vitro transcription.
Culture mammalian cells, including murine embryonic stem (ES) cells; transfect cells with electroporation/ nucleofection and lipid-mediated transfection; pick and maintain stably transfected ES clones; stain cells or slides with antibodies.
Collect early mouse embryos (zygotes and blastocysts) and inject them with DNA or cells using micro-injection techniques; implant injected embryos into pseudo-pregnant foster mothers using embryo transfer techniques (oviduct transfer and uterus transfer).
Retrieve gene structure and sequence information from genome browsers; analyze DNA sequence for searching restriction sites and the most suitable region for conducting genome engineering experiments; submit PCR fragments or plasmids for DNA sequencing; analyze the sequence data to identify mutations.
Maintain inventory of laboratory supplies; maintain laboratory equipment.
Analyze experimental data; Update and maintain laboratory notebooks and databases; collect, analyze and interpret data; collect, organize and record experimental data for publication and future reference by others; Perform computer analysis and use computer software to prepare images
Edit and write research papers and protocols; participate and collaborate in the preparation of scientific publications for peer reviewed journals based on the results.
Document information on procedures, modifications and methods for input into final publication and presentations of findings at meetings.
Work with staff on the preparation of data and images for use in research presentations and publications.
What You Will Need :
Bachelor's Degree
At least SIX (6) years of experience with conducting molecular biological experiments and/ or culturing and transfecting mammalian cells.
Experience in handling, injecting, and genotyping mice
Must be able to OBTAIN and MAINTAIN a Federal or DoD "PUBLIC TRUST"; candidates must obtain approved adjudication of their PUBLIC TRUST prior to onboarding with Guidehouse. Candidates with an ACTIVE PUBLIC TRUST or SUITABILITY are preferred.
What Would Be Nice To Have :
Experience in collecting and microinjecting early mouse embryos is highly desirable
Experience with MS Office
The annual salary range for this position is $98,000.00-$163,000.00. Compensation decisions depend on a wide range of factors, including but not limited to skill sets, experience and training, security clearances, licensure and certifications, and other business and organizational needs.
What We Offer:
Guidehouse offers a comprehensive, total rewards package that includes competitive compensation and a flexible benefits package that reflects our commitment to creating a diverse and supportive workplace.
Benefits include:
Medical, Rx, Dental & Vision Insurance
Personal and Family Sick Time & Company Paid Holidays
Parental Leave
401(k) Retirement Plan
Group Term Life and Travel Assistance
Voluntary Life and AD&D Insurance
Health Savings Account, Health Care & Dependent Care Flexible Spending Accounts
Transit and Parking Commuter Benefits
Short-Term & Long-Term Disability
Tuition Reimbursement, Personal Development, Certifications & Learning Opportunities
Employee Referral Program
Corporate Sponsored Events & Community Outreach
Care.com annual membership
Employee Assistance Program
Supplemental Benefits via Corestream (Critical Care, Hospital Indemnity, Accident Insurance, Legal Assistance and ID theft protection, etc.)
Position may be eligible for a discretionary variable incentive bonus
About Guidehouse
Guidehouse is an Equal Opportunity Employer-Protected Veterans, Individuals with Disabilities or any other basis protected by law, ordinance, or regulation.
Guidehouse will consider for employment qualified applicants with criminal histories in a manner consistent with the requirements of applicable law or ordinance including the Fair Chance Ordinance of Los Angeles and San Francisco.
If you have visited our website for information about employment opportunities, or to apply for a position, and you require an accommodation, please contact Guidehouse Recruiting at 1-571-633-1711 or via email at RecruitingAccommodation@guidehouse.com . All information you provide will be kept confidential and will be used only to the extent required to provide needed reasonable accommodation.
All communication regarding recruitment for a Guidehouse position will be sent from Guidehouse email domains including @guidehouse.com or guidehouse@myworkday.com . Correspondence received by an applicant from any other domain should be considered unauthorized and will not be honored by Guidehouse. Note that Guidehouse will never charge a fee or require a money transfer at any stage of the recruitment process and does not collect fees from educational institutions for participation in a recruitment event. Never provide your banking information to a third party purporting to need that information to proceed in the hiring process.
If any person or organization demands money related to a job opportunity with Guidehouse, please report the matter to Guidehouse's Ethics Hotline. If you want to check the validity of correspondence you have received, please contact recruiting@guidehouse.com . Guidehouse is not responsible for losses incurred (monetary or otherwise) from an applicant's dealings with unauthorized third parties.
Guidehouse does not accept unsolicited resumes through or from search firms or staffing agencies. All unsolicited resumes will be considered the property of Guidehouse and Guidehouse will not be obligated to pay a placement fee.
