Apply for Job Job ID368981 LocationTwin Cities Job FamilyResearch Full/Part TimeFull-Time Regular/TemporaryRegular Job Code8352GC Employee ClassCivil Service Add to Favorite Jobs Email this Job About the Job The successful candidate will be designated as an Essential Employee. Essential Employees are expected to report to campus during a University State of Emergency (reduced operations or campus closure) unless specifically directed by the Department not to report or if directed by civil emergency or medical authorities not to report for health and safety reasons. As an Essential Employee, you must report to campus even if the closure is less than 3 days. Responsibilities: 50% - Conduct blood and marrow transplant and anti-tumor experiments with mouse and human T cellsin support of clinical applications. A range of activities will be expected: Perform primary cell isolation and enrichment under supervision. Conduct tissue collection in a team-centered approach. Flow cytometric staining and processing. Analysis will be done be the PI, experienced lab member, or by the incumbent under direct supervision. Biochemical analyses to include DNA, RNA, and protein (immunoblot) evaluation Candidate will be expected to: Undertake standard laboratory analyses independently, carefully following detailed laboratory protocols. Perform experiments, under moderate supervision, consisting of either multiple timepoints or a variety of experimental variables. Keep accurate records of experimental outlines and results. 20% - Cell culture, team science, and reagent preparation. These duties will include: Perform basic cell culturing of standard cell lines. Undertake cloning and preparation of common bacterial and lentiviral vectors, under supervision. Maintain common stocks of media, buffers and commonly used reagents Candidate will be expected to: Work and communicate with others in a team-centered approach. Be flexible in their thinking and timing to more than one task on the same day. Practice a thorough approach to scientific execution and maintain a high internal standard of quality control. Maintain exquisite quality control of stock reagents 20% - Data review and overall lab management Diligently document experiments in lab notebooks. Prepare summary reports of experimental results to share with supervisor. Ensure lab maintenance (ordering supplies, checking inventory), including completion of regulatory paperwork as required (OSHA, IACUC, radiation safety). With direction from the PI, will implement and revise an organizational framework for the lab. This will include, but not be limited to, maintaining a spreadsheet of lab duties, ensuring proper maintenance of lab notebooks, and documenting freezer stocks and reagent inventory. Delegate shared lab duties to other members of team Other projects as assigned by supervisor. Candidate will be evaluated based upon: Ability to track lab usage of common reagents and provide replacements in a timely fashion. Timeliness in completing regulatory paperwork as well as open communication with regulatory agencies inside and outside the university. Systematic organization of the lab and proper delegation of tasks to all lab members 10% - Act as a resource for medical students and undergraduates. Help on-board new lab members Serve as a front-line resource for day-to-day general, lab-related questions (e.g., where is the media kept?). General onboarding of new lab members, including assignment of proper online manuals and modules Training of new technicians in specific areas and as directed by the PI Generally ensure that proper techniques and standardized methods are being followed across laboratory personnel. Candidate is expected to demonstrate: Open communication with multiple members of the laboratory Working knowledge of protocols and techniques that are common to the laboratory Qualifications Qualifications All required qualifications mustbe documented on application materials Required Qualifications: BA/BS in a scientific or health related field and at least 2 years of research experience OR a combination of education and experience in research to equal at least 6 years Experience working within a basic or translational science research setting and documented ability to perform experiments in a research capacity with more than one variable or time point General exposure to molecular biology and particular experience in cell culture, including the practice of aseptic technique Experience reporting effectively on the progress and direction of individual projects using written and verbal English, to include proper grammar usage and accurate spelling Demonstrated data management skills, including data collection, data entry and quality control Computer proficiency and ability to navigate basic software applications (Microsoft Word, Excel, Powerpoint) and online data storage (shared drives, google sheets, etc.) Ability to work both independently, with moderate supervision, and as part of a team Capacity to adopt flexible work hours, including occasional evenings and weekends Preferred Qualifications: Previous experience working with and handling mice in a research setting. Candidate would be expected to pick up, manipulate, and restrain mice; administer therapeutics (e.g. via intraperitoneal injection); and collect blood samples from living mice A working knowledge of immunology and murine models of disease. Previous experience working with models of in vivo tumor responses, bone marrow transplantation, or graft-versus-host disease Ability and desire to contribute to laboratory meetings and attend and participate in journal clubs Experience with flow cytometry to include staining of cells and running of samples Familiarity in ordering lab supplies and keeping a laboratory well-provisioned Excellent written, verbal, and interpersonal skills About the Department Peds BMT Byersdorfer Lab This position is located in the Department of Pediatrics, Division of Blood and Marrow Transplantation. Specifically, the applicant will be working in a laboratory that employs cellular engineering to deliver therapies for both blood and marrow transplantation and for cancer patients suffering from relapsed or refractory disease. The lab uses animal models and precise genetic engineering to modulate the therapeutic potential of various subsets of immune cells. Pay and Benefits Pay Range: $57,000 - $65,000/annually, depending on education/qualifications/experience Time Appointment Category: 100% Appointment (If job description above does not specify appointment or expected weekly hours, assume a 100% appointment.) Position Type:Civil-Service & Non-Faculty Labor Represented Staff Please visit the Office of Human Resources website for more information regarding benefit eligibility. The University offers a comprehensive benefits package that includes: Competitive wages, paid holidays, and generous time off Continuous learning opportunities through professional training and degree-seeking programs supported by the... For full info follow application link. The University recognizes and values the importance of diversity and inclusion in enriching the employment experience of its employees and in supporting the academic mission. The University is committed to attracting and retaining employees with varying identities and backgrounds. The University of Minnesota provides equal access to and opportunity in its programs, facilities, and employment without regard to race, color, creed, religion, national origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientation, gender identity, or gender expression. To learn more about diversity at the U: http://diversity.umn.edu.
